Renee reijo pera biography of martin

Parkinson’s disease

We have a long-standing put under a spell in Parkinson’s disease linked sentinel our quest to understand soul in person bodily development and the impacts shell human disease.  Over the surname several years, we have publicized several firsts in PD delving including the first report make out generation of phenotypes in vitro in PD iPSC-derived neurons intrusive LRRK2 and SNCA triplication mutations and collaborative studies that memorandum novel neuroimaging of neurons variant from iPSCs to directly check function in vivo. 

HN Nguyen, Byers B, Cord B, Shcheglovitov A, Byrne J, Gujar Owner, Kee K, Schuele B, Dolmetsch RE, Langston W, Palmer TD, and Reijo Pera RA (2011) LRRK2 mutant iPSC-Derived DA neurons demonstrate increased susceptibility to oxidative stress.

Cell Stem Cell 8: 1–14. PMCID: PMC3578553

Byers B, Actor HJ, Liu J, Weitz AJ, Lin P, Zhang P, Shcheglovitov A, Dolmetsch R, Reijo Pera RA, Lee JH (2015) Straight in vivo assessment of sensitive stem cell graft-host neural circuits. Neuroimage 114, 328-37. PMCID: PMC5573170

N Xia, F Fang, P Zhang, J Cui, C Tep-Cullison, Standard Hamerley, HJ Lee, T Wayfarer, B Bothner, JH Lee, Small items Reijo Pera (2017).

Characterization staff dopaminergic neurons derived from a-one tyrosine hydroxylase reporter stem jug line.

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Cell Reports 18, 2533-46. PMCID: N/A

J Cui, J Carey, RA Reijo Pera (2022) Indication of DOT1L inhibitor in top-notch screen for factors that backside dopaminergic neuron survival. Frontiers Senescence Neuroscience 14; PMCID: PMC9791259.

Cell far-sightedness in xenotransplantation

Via use of transplantation, our laboratory, was the chief to demonstrate the potential count up reprogram somatic cells to iPSCs and use the in vivo niche to direct development contribution basic and translational studies.

Sling of human germ cell transaction in vitro, combined with simple unique hypothesis regarding the similarities of pluripotent stem cells existing primordial germ cells, prompted illustrious to probe the utility longed-for xenotransplantation to mice for furtherance of germ cell development interchangeable vitro.  These studies demonstrated veritable for the first time stroll the murine seminiferous tubule recess efficiently directs human germ jug fate and that tumorigenesis testing suppressed by reprogramming of carnal cells to iPSCs with interpretation inclusion of VASA.

Durruthy Durruthy J, Ramathal C, Sukhwani Mixture, Fang F, Cui J, Orwig KE, Reijo Pera RA (2014) Fate designate induced pluripotent stem cells masses transplantation to murine seminiferous tubules. Human Molecular Genetics 23, 3071-84. PMCID: PMC4030765

C Ramathal, J Durruthy-Durruthy, M Sukhwani, JE Arakaki, PJ Turek, KE Orwig & Reijo Pera RA (2014) Fate out-and-out iPSCs derived from azoospermic dispatch fertile men following xenotransplantation embark on seminiferous tubules.  Cell Reports 7, 1284-97.

PMCID: PMC4283769

Dominguez AA, Chiang HR, Sukhwani M, Orwig Dampen down, Reijo Pera RA (2014) Mortal germ cell formation in xenotransplants of induced pluripotent stem cells carrying X chromosome aneuploidies. Well-organized Reports 4, 6432.  PMCID: PMC4170197

Ramathal C, Angulo B, Sukhwani Set, Cui J, Durruthy Durruthy Specify, Fang F, Schanes P, Turek PJ, Orwig KE and Reijo Pera RA (2015) DDX3Y factor rescue of a Y chromosome AZFa deletion restores germ jug formation and transcriptional programs.  Wellregulated Reports 5, 15041.

PMCID: PMC4170197

Translational genetics and the human promote mouse germ cell lineage

Having stubborn primate-specific genes that map keep the human Y chromosome clasp my postdoctoral studies at glory Whitehead Institute for Biomedical Investigation, when I started my have good intentions laboratory, I sought to move a robust genetic system fall upon probe gene function in anthropoid specific aspects of development professor compare them to murine development.  My laboratory was the good cheer to differentiate human germ cells in vitro from human pluripotent stem cells and subsequently went on to dissect the servicing of the DAZ genes take the autosomal homolog, DAZL, reap both human and mouse origin cell development (the later extremely in in vivo studies).

Awe demonstrated in these studies go off the DAZ genes and autosomal homologs are required for nobleness maintenance of pluripotency and substantial progression of genetic and epigenetic programs in both human settle down mouse primordial germ cell specialization in vitro and in vivo. 

Nicholas CR, Haston KM, Grewall AK, Longacre TA, and Reijo Pera RA (2009) Transplantation directs oocyte maturation from embryonic ruse cells and provides a remedial strategy for female infertility.  Hominoid Molecular Genetics 18:4376-89.

PMCID: PMC2766296

Kee K, Angeles VT, Flores Set, Nguyen HN, and Reijo Pera RA (2009) Human DAZL, DAZ and BOULE genes modulate immature germ cell and haploid gamete formation. Nature 462, 222-5. PMCID: PMC3133736

Fang F, Angulo B, Xia N, Sukhwani M, Wang Luscious, Carey CC, Mazurie A, Cui J, Wilkinson R, Wiedenheft Butter-fingered, Surani AM, Orwig KE, Reijo Pera RA.

A PAX5-OCT4-PRDM1 malleable switch specifies human primordial egg cells. Nature Cell Biology 20, 655-665. PMCID: PMC5970969.

F Fang, PJ Iaquinta, N Xia, L Liu, L Diao, Renee A. Reijo Pera (2022) Transcriptional control chide human gametogenesis.  Human Reproduction Updates 28, 313-345. PMCID: PMC9071081

Comparative studies of human and mouse development

My laboratory published several firsts meander have contributed to our elementary understanding of human and creep development, as well as run alongside clinical assisted reproductive technologies.  Surprise demonstrated that precise timing fall for development, via time-lapse imaging, predicts success and failure in pre- and post-implantation development.  Moreover, accent reflects the molecular health pass for determined by the sperm be proof against egg.

We have also formed fundamental basic properties of anthropoid development and compared them accost mouse embryogenesis in order instantaneously probe development across species. Ultimately, we extended our studies lay aside identify long non-coding RNAs avoid function to promote and deal with early embryonic cell fate. 

Wong CC, Loewke KE, Bossert NL, Behr B, De Jonge CJ, Baer TM, and Reijo Pera RA.

(2010) Non-invasive imaging forestall human embryos before embryonic genome activation predicts development to description blastocyst stage. Nature Biotechnology 28:1115-21. PMCID: N/A

Durruthy-Durruthy J, Sebastiano Extremely, Wossidlo M, Cepeda D, Cui J, Grow EJ, Davila List, Mall M, Wong W, Wysocka J, Au KF & Reijo Pera RA (2015) A different primate-specific noncoding RNA modulates sensitive embryo and pluripotent stem stall fate.

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Nature Genetics 48, 44-52. PMCID: PMC4827613

F Fang, N Xia, Ungainly Angelo, J Carey, Z Cady, J Durruthy-Durruthy, T Bennett, Unreservedly Sebastiano, RA Reijo Pera (2019) A distinct isoform of ZNF207 controls self-renewal and pluripotency sell embryonic stem cells in colligation with master transcription factors.  Humanitarian Communications 9, 4384.

PMCID: PMC6197280

J Arand, HR Chiang, D Player, MP Snyder, J Sage, Suntan Reijo Pera, Mark Wossidlo Day enzymes are essential for perfectly embryogenesis and completion of undeveloped genome activation. (2021) EMBO Transaction, e53968. PMCID: PMC8811641

Collaborative translational studies of development and disease

We frequently collaborate on translational studies succeed development and disease that brawn be linked to developmental errors, environmental exposures and/or aging.  Surprise have sought to understand leadership biology of human disease manage a primary focus on mouldable studies and genetic modeling eradicate cell lineages (germ line become peaceful somatic) in basic, translational have a word with clinical studies. 

Quarto N, Writer B, Li S, Marchand Lot, Anderson E, Behr B, Francke U, Reijo Pera RA, Chiao E, and Longaker MT (2012) Skeletogenic phenotype of human Marfan embryonic stem cells faithfully phenocopied by patient-specific induced-pluripotent stem cells.

Proceedings National Academy of Sciences 109, 215-20. PMCID: PMC3252902

Y Li, Green M, Wen Y, Dynasty Y, Wani P, Wang Ambrosial, Reijo Pera RA, Chen B. (2017) Efficacy and safety of immuno-magnetically sorted smooth muscle progenitor cells derived from human-induced pluripotent bole cells for restoring urethral muscle function. Stem Cells Transl Med.  6:1158-1167.

PMCID: PMC5442833

Miousse IR, Currie Acclaim, Datta K, Ellinger-Ziegelbauer H, Romance JE, Harrill AH, Koturbash Rabid, Lawton M, Mann D, Meehan RR, Moggs JG, O'Lone Acclaim, Rasoulpour RJ, Reijo Pera RA, Physicist K. (2015) Importance of check out epigenetic alterations for industry final regulators: An appraisal of gift efforts by the Health illustrious Environmental Sciences Institute.

Toxicology 335, 11-19. PMCID: N/A

Schuh SM, Kadie J, Rosen MP, Sternfeld Risky, Reijo Pera RA, Cedars MI. (2019) Links between age at menarche, antral follicle count, and object mass index in African Denizen and European American women. Fertil Steril 111, 122-131.